Proteomics, Protein Analysis and Validation
Welcome to Proteome Factory AG!
Your specialist in contract research, fee-for-service and products for proteomics, biomarker & target discovery and validation, protein identification and characterization with more than a dozen years experience in modern protein analysis and proteomics.
We offer fully supported contract research, fee-for-services and advanced products for gel-based and gel-free proteome analysis (proteomics), protein identification, protein & peptide sequencing, characterization, analysis and epitope mapping by MS.
State-of-the-art technologies and methods are routinely employed for differential proteomics studies and quantification and validation of regulated proteins, biomarkers and targets. Beside extreme high resolution 2D electrophoresis allowing the separation of theoretically up to 10000 thousand protein spots in 40 x 30 cm 2DE gels we apply gel-free methodes like iTRAQ™* proteomics, label-free nanoLC-ESI-MSMS based proteomics and MeCAT proteomics. The later is very useful for pharmacokinetics of protein drugs allowing ultrasensitive absolute quantification of intact MeCAT lanthanide labelled proteins down to low attomol range of protein by ICP-MS. Furthermore protein phosphorylation and naturally occurring metalloproteins can be analyzed by quantification of phosphorus or other elements using ICP-MS in parallel. In addition we employ quantitative mass spectrometry of proteins and peptides by selected reaction monitoring (SRM) or multiple reaction monitoring (MRM) assays (mass western) for biomarker validation and quantification of protein expression, protein drug concentration in plasma and absolute quantification of proteins and their isoforms. In plasma proteomics and CSF proteomics we deplete high abundance proteins by affinity removal before applying 2DE or gel-free proteomics methods.
Standard and high resolution mass spectrometry is employed for epitope mapping of antibodies and identification, quantification and characterization of proteins and peptides including determination of phosphorylation and glycosylation sites and optionally their structure.
Proteins and peptides are identified with conventional database search engines or by de novo peptide sequencing using Proteome Factory’s proprietary and leading PWB Protein Identification Cluster Software Solution.
Beside proteomics studies Proteome Factory is specialized in providing full services for complete protein sequencing and characterization using a combination of N-terminal and internal Edman protein sequencing, HPLC, MSMS mass spectrometry and de novo peptide sequencing.
Proteome Factory’s quality management ensures contemporary, high quality and reproducible standard analyses. The SOPs (standard operating procedures) are applied to all standard analyses in fee-for-services as well as research projects.
Proteome Factory is proud to have worldwide customers and partners including leading pharmaceutical, food and biotech companies as well as many well-known academic institutions like the Max-Planck and Riken institutes.
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MeCAT proteomics and protein quantification MeCAT - Metal Coded Tagging - Technology for quantitative proteomics and ultrasensivite absolute protein quantification down to attomol.
Proteome Factory AG
Phone:+49 30 20616265
FAX:+49 30 20616267
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*iTRAQ™ is a trademark of Applera Corporation or its subsidiaries.