Service - Quality Control of Proteins

Proteome Factory offers the following standard analytical service portfolio for quality control of peptides, recombinant proteins, protein drugs and monoclonal antibodies.
Please contact us, if you need specific other protein analytical methods, advanced protein analysis, the development of new methods and techniques to fulfil your requirements.

Method Aim of analysis 
nanoESI-MS  Determination of molecular weight  
MALDI-MS  Determination of molecular weight up to ca. 100 kDa and more (on request) 
MS analysis of monoclonal antibody  Determination of molecular weight of the light and heavy chain 
FT-ICR-ESI-MS   High resolution determination of molecular weight (accuracy in low ppm range) 
SDS-PAGE  Determination of protein mass by using MW standard proteins in SDS-PAGE  

Determination of molecular weight of proteins

 

MALDI-MS (up to 100 kDa) with desalting

Order No.  

 price per sample [EURO]
standard service
(1-2 weeks)  		 Order No.   price per sample [EURO]
express service
(2-4 days)  
1 sample S011001 250 S011001E 330
2 samples S011002 200 S011002E 260
3 - 4 samples S011003 150 S011003E 200
5 - 9 samples S011005 125 S011005E 165
10 and more samples S011010 100 S011010E  
MALDI-MS of antibody (incl reduction and desalting) for analysis of heavy and light chain S011100 300 S011100E 400
         
high resolution ESI-MS with desalting

Order No.  

 price per sample [EURO]
standard service
(1-2 weeks)  		 Order No.   price per sample [EURO]
express service
(2-4 days)  
1 sample S021001 upon request S021001E upon request
2 samples S021002 upon request S021002E upon request
3 - 4 samples S021003 upon request S021003E upon request
5 - 9 samples S021005 upon request    
10 and more samples S021010 upon request    
         


Determination of amino acid sequence of proteins

Analysenmethode   Zielstellung   Nettopreis pro Analyse (zzgl. 19% MwSt.)  
N-terminal sequencing   Analysis of the N-terminal amion acid sequence and homogenity of the N-terminus  see Edman sequencing
Protein sequencing  Analysis and validation of the primary amino acids sequence of complete proteins by mass spectrometry as well as N-terminal and internal Edman protein sequencing  On request 
Protein identification and peptide mapping  Conformation of protein identity and detectable peptides by nanoLC-ESI-MSMS after tryptic protein digest  see protein identification
Analysis of disulfid bridges  Conformation and determination of the position of disulfid bridges  On request 
Analysis of post-translation modifications  Detection and conformation of sequence position of post-translational modification  On request 


Determination of purity and impurities

Analysenmethode   Zielsetzung   Nettopreis pro Analyse (zzgl. 19% MwSt.)  
2D electrophoresis, SDS-PAGE, western blot, MeCAT  Determination of homogenity and purity of a product or batch; detection and quantfication of isoforms and protein species   On request 
2D electrophresis and protein identification  Determination of homogenity and purity of a product or batch as well as identification of proteins, protein contaiminations and degradation products  On request 
Chromatography  Determination of homogenity and purity of protein and peptide products or batches  On request 
Bradford assay for protein concentration determination  Determination of protein concentration in a product or batch  On request 
MeCAT protein quantification by MeCAT labeling and ICP-MS  Absolute ultra sensitive and exact protein quantification in a product or batch  On request